Limbal Stem Cell Deficiency (LSCD) Treatment With Cultivated Stem Cell (CALEC) Graft

Inclusion Criteria: * Male or female participants age 18 to \<90 years old at time of enrollment * Ability of a subject or guardian/legal representative to provide written informed consent and to comply with study assessments for the full duration of the study. * Patients with unilateral limbal stem cell deficiency (LSCD) as determined by conjunctivalization of the cornea defined by fibrovascular pannus more than 2 mm from the limbus for greater than or equal to 6 clock hours. * Additional optional criteria: * Lack of limbal palisades of Vogt for greater than or equal to 9 clock hours * Goblet cell presence as defined by impression cytologic criteria Exclusion Criteria: * Corneal or ocular surface infection within 30 days prior to study entry or CALEC transplantation * Ocular surface malignancy * Uncontrolled diabetes with most recent HgA1c greater than 8.5% * Renal Failure with eGFR below 60 mL/min per 1.73 m2 * Aspartate aminotransferase and alanine aminotransferase levels greater than 3 times institutional upper limit of normal * Total bilirubin greater than 2 times institutional upper limit of normal (except patients with known Gilbert's syndrome) * Platelet levels less than 100,000 or greater than 450,000 per microliter * Hemoglobin levels of less than 11.0 g/dL in men or less than 10.0 g/dL in women * Prothrombin time greater than 16 seconds and activated partial thromboplastin time greater than 35 seconds in patients not taking warfarin and an international nor…