Evaluation of Efficacy and Safety of Autologous MSCs Combined to Biomaterials to Enhance Bone Healing

Inclusion Criteria: * Age 18 to 65, both sexes * Traumatic isolated closed or open Gustilo I and II humerus, tibial or femur diaphyseal or metaphyseal-diaphyseal fracture status delayed union or non-union * At least 3 months from acute fracture * Able to provide informed consent, and signed informed consent * Patients (by themselves) should have medical health care coverage to be included in a research study * Able to understand and accept the study constraints Exclusion Criteria: * Pregnancy, breast feeding women and women who are of childbearing age and not practicing adequate birth control * Participation in another therapeutic trial in the previous 3 months * Delayed union or non-union related to iatrogeny * Segmental bone loss requiring specific therapy (bone transport, large structural allograft, megaprosthesis, etc) * Vascular or neural injury * Other fractures causing interference with weight bearing * Acute persistent chronic bacterial infections such as brucellosis, typhus, leprosy, relapsing fever, melioidosis and tularemia * Visceral injuries of diseases interfering with callus formation (cranioencephalic trauma, etc.) * History of bone harvesting on iliac crest contraindicating bone-marrow aspiration * Corticoid or immunosuppressive therapy more than one week in the three months prior to study inclusion * History of prior or concurrent diagnosis of HIV-, Syphilis, Hepatitis-B- or Hepatitis-C-infection (confirmed by serology or PCR) * History of neoplasia or cu…