Characterisation of T Lymphocytes, NK Cells and Macrophages in Melanoma Patients

Inclusion Criteria: * Patients with confirmed melanoma-patients informed of the aims of the study , the modalities * Patients who signed the consent form ou a non opposition form signed by the patient or by a relative According to study part: Part 1: patients with a melanoma larger than 10mm or with cutaneous metastasis Part 2: stage IV melanoma patients who shall undergo a regional lymph node dissection Part 3: retrospective study of patients who underwent a sentinel node procedure Part 4: Patients achieves of inoperable melanomas the stage(stadium) III or IV that must receive in treatment (processing) a chemotherapy (by the dacarbazine or by the cisplatin or by the inhibitor of B-raf) and carrier of at least two cutaneous metastases Exclusion Criteria: * Refusal to take part in the study (patient or relative) * Contraindications known to the xylocaine (groups 1,2, and 4) * No affiliated to the social security system (groups 1, 2, and 4)