Alternate Day Fasting for Weight Loss, Weight Maintenance and Cardio-protection

Inclusion criteria. Adult subjects meeting the following criteria will be eligible to participate: * Age between 18 to 65 years old * BMI between 25.0 and 39.9 kg/m2 * Previously sedentary (\<60 minutes/week of light activity corresponding to 2.5 to 4.0 metabolic equivalents (METs) for the 3 months prior to the study) Exclusion criteria. Subjects excluded from participating in the study include those who: * Have a history cardiovascular disease (prior angina, myocardial infarction or stroke) * Are diabetic (fasting blood glucose \> 126 mg/dl) * Have a history of psychiatric disorders and/or eating disorders * Are taking anti-depressant or anti-anxiety medications * Are taking drugs that affect study outcomes (weight loss, lipid-lowering, or blood pressure drugs) * Are not weight stable for 3 months prior to the beginning of study (weight gain or loss \> 4 kg) * Are not able to keep a food diary or activity log for 7 consecutive days during screening * Are perimenopausal or have an irregular menstrual cycle (menses that does not appear every 27-32 days) * Are claustrophobic or have implanted electrical devices (cardiac pacemaker or a neurostimulator) * Are pregnant, or trying to become pregnant * Are smokers